Structure and Function of Polarized Epithelial Cells
A one-week laboratory short course in comparative physiology for first year medical students from the University of Pittsburgh School of Medicine
| Date: | May 22-29, 2010 | |
| Location: | Mount Desert Island Biological Laboratory |
Overview
The course will utilize marine animal models to elucidate mechanisms of human physiology through a variety of biomedical research techniques. It will consist of both lectures and hands-on training in the laboratory.
Intensive one-week course in biomedical research protocols and techniques is open to first-year medical students from the University of Pittsburgh School of Medicine. Four biomedical research topics are the focus of this course:
- physiological studies of chloride transport in an intact epithelial organ from Squalus acanthias;
- ion channel gene expression in a heterologous expression system (Xenopus oocytes);
- studies in isolated tubule preparations, including immunocytochemistry of phosphorylated vs. non-phosphorylated co-transporters, tissue processing, confocal microscopy, Western blots, and antibody design;
- molecular biology of membrane proteins and transporters in shark salt gland, including methods in RNA, cDNA, PCR, cloning, and sequencing.
Ray Frizzell, Ph.D., Course Director
Professor and Chair, Cell Biology and Physiology
University of Pittsburgh School of Medicine
John N. Forrest, Jr., M.D.
Professor of Medicine
Director, Office of Student Research
Yale University School of Medicine
Will Walker, Ph.D.
Associate Professor, Cell Biology and Physiology
University of Pittsburgh School of Medicine
Simon C. Watkins Ph.D., FRCPath
Professor and Vice Chair, Cell Biology and Physiology
Professor, Immunology
Director, Center for Biologic Imaging
| Saturday | |
|---|---|
| 1:00 PM | Arrival |
| 6:00 PM | Dinner, Co-Op |
| 7:30 PM | All Groups - Orientation and Opening Talk - Maren Auditorium, MDIBL Introduction of Labs - Rotations A-D |
| Sunday | |
| 8:30 AM, PM, and Evening | Rotations A1, B1, C1, D1 |
| Monday | |
| 8:30 AM | Rotations A1, B1, C1, D1 |
| 10:00 AM | All Groups - Small Lab Meetings (by Rotation) |
| 12:00 Noon | Lunch |
| 12:30 PM | Large Lab Meeting |
| 2:30 PM | Free Time |
| Tuesday | |
| 8:30 AM, PM & Evening | Rotations A2, B2, C2, D2 |
| Wednesday | |
| 8:30 AM | Rotations A2, B2, C2, D2 |
| 10:00 AM | All Groups - Small Lab Meetings (by Rotation) |
| 12:00 Noon | Lunch |
| 12:30 PM | Large Lab Meeting |
| 2:30 PM | Free Time |
| 7:30 PM | LECTURE |
| Thursday | |
| 8:30 AM, PM and Evening | Rotations A3, B3, C3, D3 |
| Friday | |
| 8:30 AM | Rotations A3, B3, C3, D3 |
| 10:00 AM | All Groups - Small Lab Meetings (by Rotation) |
| 12:00 Noon | Lunch |
| 12:30 PM | Large Lab Meeting and Evaluation |
| 2:30 PM | Free Time |
| 6:00 PM | Lobster Fest at Seawall - Acadia National Park |
| Saturday | |
| 8:00 AM | YOU MUST BE CHECK OUT OF YOUR ROOM BY 8:30AM. Luggage can be stored in the Dining Hall until departure. |
Housing: Medical students will be assigned to a room in either Spruce or Birch Hall.
Travel: Travel is coordinated through the University of Pittsburgh School of Medicine.
Driving directions and a campus map are available on the MDIBL Travel Information page.
The Bar Harbor Chamber of Commerce has information regarding local attractions, including Acadia National Park.