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Quantitative Fluorescence Microscopy

A national techniques course in fluorescence microscopy and imaging

Date:  May 16–22, 2014
Location:   MDI Biological Laboratory

Overview

fluorescence

Applications for 2014 are now closed.

This one week, intensive microscopy course will cover all aspects of the technology from the principals of fluorescence imaging to multidimensional imaging in living cells. Lectures dealing with the theory, mechanics, and application of fluorescent imaging methods will be intermingled with extended laboratory practicals in which students will be encouraged to use their own specimens, to optimize the utility of the course. Our goal will be to provide students with the knowledge and expertise to implement cutting edge microscopic methods within their own laboratories.

Contact

Simon C. Watkins, Ph.D., Course Director
Professor, Cell Biology & Physiology
University of Pittsburgh School of Medicine
412-648-3051
swatkins@pitt.edu

 

Judi Medlin
Education Coordinator
207-288-9880 x102

Tuition

$3,100 (room and board included)

Rationale

The power and capability of the light microscope has increased dramatically over the last few years. In fact light microscopy is now becoming of central utility in all fields of biological research including cell biology, physiology and molecular biology. This change has been driven by the need to study increasingly sophisticated problems at high spatial and temporal resolution. The advances, principally in the field of fluorescence microscopy have been dependant on the development of entirely new microscopic methodologies coupled to the use of fluorescent proteins, new fluorescent dye technologies, highly sensitive detectors and inexpensive powerful computers.

Our goal will be to provide students with the knowledge and expertise to implement cutting edge microscopic methods within their own laboratories. In contrast with other currently available courses it will focus exclusively on the theory, potential application and implementation of fluorescent imaging techniques, and perhaps more importantly this will be couched in the real world application of biological experimentation.

This one week, intensive course will cover all aspects of the technology from the principals of fluorescence imaging to multidimensional imaging in living cells. Lectures dealing with the theory, mechanics, and application of fluorescent imaging methods will be intermingled with extended laboratory practicals in which students will be encouraged to use their own specimens, to optimize the utility of the course.

Teaching Faculty

Simon C. Watkins, Ph.D.
Professor, Cell Biology & Physiology
University of Pittsburgh School of Medicine
Pittsburgh, PA 15261 USA
Phone:(412)648-3051
Fax: 648-8330
swatkins@pitt.edu

David W. Piston, Ph.D.
Professor of Molecular Physiology and Biophysics
Associate Professor of Physics
Vanderbilt Medical Center
1211 22nd Ave S
Nashville, TN 37232

Claudette M. St. Croix, Ph.D.
Assistant Professor
Department of Environmental and Occupational Health University of Pittsburgh Graduate School of Public Health
s220 BST 3500 Terrace Street
Pittsburgh, PA 15219

Anne Kenworthy, Ph.D.
Assistant Professor
Vanderbilt University
Nashville, TN

Gert-Jan Kremers, Ph.D.
Imaging Centre Erasmus MC
Rotterdam, The Netherlands

Industrial Faculty

Intelligent Imaging
Leica
Nikon
Carl Zeiss
Chroma
Glass Bottom Dishes

Friday, May 16, 2014

16:00 Arrival on campus, check-in to housing, get keys at Maren Conference Center

18:00 Dinner and Social at Co-Op

19:30 Reception at Maren Conference Center and conference material pickup

Late arrival keys placed in Dining Hall keybox for pick-up

 

Saturday, May 17, 2014

anytime self-serve breakfast in the dining hall

09:00-09:30 Introduction Welcome, Introductions, Define Course Goals (Piston and St. Croix)

09:30-10:30 The Microscope 1 Advances in Modern Imaging for Biological Problems (Kenworthy)

10:30-11:00 Break at Maren Conference Center

11:00-12:00 The Microscope 2 Transmitted Light Contrast in Microscopy (Piston)

12:00-13:00 Lunch

13:00-14:45 LAB 1 TRANSMITTED LIGHT MICROSCOPY

15:00-15:45 The Microscope 3 Fluorescence Microscopy and Quantitation (Piston)

15:45-16:45 Live Cell Imaging 1 Green-Fluorescent Protein (Kremers)

16:45-17:00 Break

17:00-18:00 Digital Imaging 1 Digital Imaging, Resolution, and Detectors (Watkins)

18:00-19:00 Dinner

19:00-20:00 Digital Imaging 2 Optical Filters and Brightness Demonstration (Watkins et al.)

20:30-23:00 LAB 2 STANDARD FLUORESCENCE MICROSCOPY

 

Sunday, May 18, 2014

anytime self-serve breakfast in the dining hall

09:00-09:45 Optical Sectioning 1 Introduction to the Confocal Microscope (St Croix)

09:45-10:30 Optical Sectioning 2 Confocal Detectors and “Pseudo-confocals” (Piston)

10:30-11:00 Break at Maren Conference Center

11:00-12:00 Emerging Methods 1 Super-Resolution (Kenworthy)

12:00-13:00 Lunch

13:00-16:30 LAB 3 OPTICAL SECTIONING MICROSCOPY

16:30-17:30 Tips & Tricks Digital Filters (Watkins)

18:00-19:00 Dinner

19:00-23:00 LAB 4 IMAGE PROCESSING & FEATURE EXTRACTION

 

Monday, May 19, 2014

anytime self-serve breakfast in the dining hall

09:00-09:45 Image Processing 1 Practical Digital Imaging (Watkins)

09:45-10:30 Image Processing 2 Considerations for Image Quantitation (Piston)

10:30-11:00 Break at Maren Conference Center

11:00-12:00 Optical Sectioning 3 3D Methods, Microfluorimetry, Spectral Imaging (St Croix)

12:00 noon Course Photo

12:00-13:00 Lunch

13:00-16:00 LAB 5 MULTICOLOR 3-D IMAGING

16:00-17:30 Tips & Tricks Cell Labeling, Antibodies, Practical Aspects (Watkins)

Evening off Dinner on your own (car pools/van to Bar Harbor will be available)

 

Tuesday, May 20, 2014

anytime self-serve breakfast in the dining hall

09:00-10:00 Live Cell Imaging 2 FRAP, Photoactivation, TIRF (Kenworthy)

10:00-10:30 Emerging Methods 2 Light Sheet, SPIM, direct SIM (Piston)

10:30-11:00 Break at Maren Conference Center

11:00-12:00 Optical Sectioning 4 Deconvolution (Piston)

12:00-13:00 Lunch

13:00-17:00 LAB 6 DECONVOLUTION, SUPER-RESOLUTION

17:00-18:00 Tips & Tricks Live Cell Imaging Design (Watkins)

18:00-19:00 Dinner

19:00-23:00 LAB 7 TIRF, FRAP, PHOTOACTIVATION

 

Wednesday, May 21, 2013

anytime self-serve breakfast in the dining hall

09:00-09:45 Live Cell Imaging 3 Multicolor, Ratio Imaging (St Croix)

09:45-10:30 Optical Sectioning 5 Multi-Photon Microscopy (Piston)

10:30-11:00 Break at Maren Conference Center

11:00-12:00 Live Cell Imaging 4 FRET and Live Cell Imaging (Kremers)

12:00-13:00 Lunch

13:00-17:00 LAB 8 LIVE CELL FRET IMAGING

17:00-17:30 LAB 9 SETUP COLLABORATIVE GROUPS FOR LAB 10 BASED ON STUDENT INTERESTS

18:00-19:00 Dinner

19:00-23:00 LAB 10 TIME-LAPSE LIVE CELL IMAGING, TRACKING, QUANTITATION, RATIO IMAGING METHODS, STUDENT EXPERIMENTS

 

Thursday, May 22, 2013

anytime self-serve breakfast in the dining hall

09:00-10:00 Image Processing 4 Advanced Image Processing (Watkins)

10:00-10:30 Break at Co-Op

10:30-14:00 LAB 11 ADVANCED IMAGE PROCESSING: 3D QUANTIFICATION, PARTICLE TRACKING; DATA COLLECTION AND PRESENTATION

12:00-13:00 Lunch

14:00-16:00 Results! Student Presentations

16:00-16:30 Mechanics How do I get money to pay for all this stuff? (Watkins)

16:30-17:00 Summing up Q&A, Student Feedback (Faculty)

17:30 Lobster bake Caravan to Thompson Island Picnic Area

 

Friday, May 23, 2013

Move-out of housing units no later than 9:00am.

Housing: On-campus housing is available in single and double-accommodation dormitory rooms and cottages. The course fee includes the cost of on-campus housing.

Travel, Shuttles, and Public Transportation: Detailed travel information, including airport and ground transportation suggestions, is available on the directions page.

Most course participants will be housed on-campus and daily transportation will not be necessary.

The Bar Harbor Chamber of Commerce has information regarding local attractions, including Acadia National Park.

Full Tuition fellowships have been generously provided by Chroma Technologies, Nikon, Carl Zeiss Microimaging, and the Maine INBRE.

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